
Featured Product: NK Cells (Cat. NK-01)
NK (Natural Killer) cells are innate immune cells with cytolytic activity against cells under stress such as tumor cells and virus-infected cells. They represent 5-15% of peripheral blood lymphocytes. NK cells are identified by expression of CD56 and lack of expression of CD3.
IGNYTE BIO’s NK cells (cat. NK-01) are >85% CD56+CD3- as shown in Figure 1. This immunophenotyping data also reports percentage of other cell types (CD14+, CD19+, or CD3+) present in the cell product.
Figure 1. Immunophenotyping shows NK cell purity. Quadrant positions were determined based on the respective isotype control staining.

These cryopreserved primary NK cells maintain their cytotoxic activity against K562, a known NK target cell type, when used right after thawing. Figure 2 depicts the extent of K562 cytolysis at different Effector to Target (E:T) ratios by NK cells from two donors. These numbers are comparable to data from freshly isolated NK cells.
Figure 2. Cytotoxicity of primary NK cells against K562. NK cells from two donors were thawed and cultured with K562 at different E:T ratios for 16-20 hours prior to addition of 7-AAD viability dye. % dead K562 values are plotted against E:R ratio.

Contact us for information and ordering via email:
Browse our product page.