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IGNYTE BIO offers the highest quality biological reagents (including human immune cells, antigens and other reagents) to support your research, product and assay developments. Our products are “ Made by Scientists for Scientists”, backed by a team with diverse background and experience in immune cell isolation and culture, as well as research in immunology and assay development.

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New Inventory

We have added new inventory of cryopreserved immune cells, including PBMC, as well as individual cell components from PBMC, such as: NK cells, pan CD3 T cells, CD4 and CD8 T cells, and monocytes. These “untouched” cell types were enriched by negative selection to assure that they maintain their natural state in the blood. Class I and II HLA-typing, FcgammaR3a-158 genotype, and CMV serological status is included with each donor information.

IgnyteBio - PBMC
Ignyte Bio - NK Cells
Ignyte Bio - T Cells
Monocytes
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Featured Product: NK Cells

NK cells are large granular lymphocytes that are a big player in innate immune response. IGNYTE BIO offers cryopreserved negatively selected NK cells from normal donors. We characterized these cell products to examine the purity and functional activity.

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Featured Product: NK Cells (Cat. NK-01)

NK (Natural Killer) cells are innate immune cells with cytolytic activity against cells under stress such as tumor cells and virus-infected cells. They represent 5-15% of peripheral blood lymphocytes. NK cells are identified by expression of CD56 and lack of expression of CD3.  

IGNYTE BIO’s NK cells (cat. NK-01) are >85% CD56+CD3- as shown in Figure 1. This immunophenotyping data also reports percentage of other cell types (CD14+, CD19+, or CD3+) present in the cell product.

Figure 1. Immunophenotyping shows NK cell purity. Quadrant positions were determined based on the respective isotype control staining.

These cryopreserved primary NK cells maintain their cytotoxic activity against K562, a known NK target cell type, when used right after thawing. Figure 2 depicts the extent of K562 cytolysis at different Effector to Target (E:T) ratios by NK cells from two donors. These numbers are comparable to data from freshly isolated NK cells.

Figure 2. Cytotoxicity of primary NK cells against K562. NK cells from two donors were thawed and cultured with K562 at different E:T ratios for 16-20 hours prior to addition of 7-AAD viability dye. % dead K562 values are plotted against E:R ratio.